FORMULATION AND IN VITRO CHARACTERISATION OF GLUCOSE-RESPONSIVE NANOCAPSULES FOR THE DELIVERY OF M-INSULIN

Objective: The present study aimed to develop and characterize Chitosan coated Alginate Nanocapsules loaded with M-Insulin Concanavalin A Complex for glucose-responsive delivery. Methods: Preformulation studies were performed on the Insulin human recombinant prepared by ionic gelation method chitosan using electrostatic attraction. formulation variables optimized Box-Behnken design (BBD) help of Design-Expert® Software. Three independent taken concentration (A1), sodium alginate (A2), stirring rate (A3). response selected average particle size (nm) (B1), polydispersity index (B2), cumulative release (%) (B3). Results: results from indicated that received sample was pure. nanocapsules possessed an 382.4 nm, PDI 0.211 zeta potential 30.25 mV. entrapment efficiency found be 79.2 %. further characterized their surface morphology TEM regular shape. in vitro drug able 58 % M-insulin hyperglycaemic conditions 12 h. Conclusion: outcomes demonstrated developed can effectively used delivery M-insulin.